Understanding the Differences Between EnGen Spy Cas9 NLS and EnGen Lba Cas12a (Cpf1)
Understanding the Differences Between EnGen Spy Cas9 NLS and EnGen Lba Cas12a (Cpf1) Introduction The CRISPR genome-editing system has revolutionized molecular biology, and two widely used nucleases in the field are EnGen Spy Cas9 NLS ( NEB #M0646 ) and EnGen Lba Cas12a (Cpf1) (NEB # M0653 ). While both enzymes require a guide RNA (gRNA) for target recognition, they exhibit fundamental differences in gRNA length, PAM sequence preferences, cleavage mechanisms, and the nature of DNA cuts. This article provides an in-depth comparison to help researchers select the appropriate enzyme for their applications. Key Differences Between EnGen Spy Cas9 NLS and EnGen Lba Cas12a (Cpf1) These differences impact how each enzyme is used in genome-editing experiments, from target specificity to cleavage efficiency and downstream applications. Understanding Guide RNA (gRNA) in EnGen Lba Cas12a (Cpf1) Both enzymes require a guide RNA (gRNA) to recognize and cleave target DNA. However, EnGen Lba Cas12a (C...